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Alternatively, the stock tradition organisms may be developed in a suitable liquid medium (i.e., Soybean–Casein Digest Broth or Sabouraud Dextrose Broth) and also the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to get a microbial count of about one × 108 cfu for every mL. [Notice—The estimate of in